What Class Of Transposon Is Phage Mu
- With a single transposon copy, it contains two copies of the transposon. The replicative nature of Mu transposition was evident from the fact that replication of the phage during a lytic in- fection was absolutely dependent upon its transposition to many sites in the genome of a single cell. The short seg.
- Mu Phage As a Giant Transposable Element A transposon could carry DNA with phage-like functions rather than a simple antibiotic resistance gene. A transposon could carry DNA with phage-like functions rather than a simple antibiotic resistance gene.
- Transposons Definition
- What Class Of Transposon Is Phage Mu Test
- What Class Of Transposon Is Phage Mu C
Transposable phage Mu has played a major role in elucidating the mechanism of movement of mobile DNA elements. The high efficiency of Mu transposition has facilitated a detailed biochemical dissection of the reaction mechanism, as well as of protein and DNA elements that regulate transpososome.
The three main examples of transposons are as follows:
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1. Tn 3 transposon of E. coli:
The molecular structure of transposon Tn 3 of E. coli has been worked out (Fig. 41.4). Tn 3 has 4957 bp and contains three genes such as tnp A, tnp R and bla, coding respectively for the following proteins: 1. transposase having 1015 amino acids and required for transposition; 2. a repressor (also called resolvase) containing 185 amino acids which regulates the transposase; and 3. β- Lactamase enzyme which confers resistance to the antibiotic ampicillin. On both sides of the Tn 3 is an inverted repeat of about 38 bp.
2. Bacteriophage Mu:
The bacteriophage Mu (.Mu = mutator) is a temperate bacteriophage having typical phage properties and could be regarded as a giant transposon. Phage Mu was thought to behave in a unique manner since it does not multiply in a lytic way. It always starts its life-cycle by inserting itself into the E. coli chromosome at random locations. The resulting prophage often mutates the genes into which they become inserted (Fig. 41.5). Thus, phage Mu was originally known for its mutagenic properties.
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During later part of phage life cycle, upon receipt of an appropriate signal, the Mu prophage somehow generates its transposition, but also activates the genes encoding for structural proteins which package its DNA. Mu phage is unique also in transposing much often than other transposons, providing nearly a hundred new copies of Mu during its hour-long life-cycle.
Transposons Definition
Like other transposons, Mu phage has inverted repeats (IR) at or near the ends of its DNA and it makes a short duplication with 5 base pairs of the bacterial DNA into which it is inserted.
3. Yeast Ty elements:
What Class Of Transposon Is Phage Mu Test
The yeast Saccharomyces cerevisiae carries about 35 copies of a transposable element called Ty in its haploid genome. These transposons are about 5900 base pairs long and are bounded at each end by a DNA segment called the delta (8) sequence, which is approximately 340 base-pairs long.
Each 8 sequence is oriented in the same direction, forming what are known as direct long terminal repeats or LTRs. Ty elements are flanked by five base-pair direct repeats created by the duplication of DNA at the site of the Ty insertion. These target site duplications are found rich in A-T base pairs. Yeast Ty elements are sometimes called retrotransposons because of their overall similarity to the retroviruses.
What Class Of Transposon Is Phage Mu C
Like the yeast, in other eukaryotes such as Zea Mays, Drosophila, mice and snapdragons (Antirrhinum majus) transposable elements of one or other type are found.